hrp conjugated secondary antibodies (Proteintech)
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Proteintech
hrp conjugated secondary antibodies
Hrp Conjugated Secondary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 5898 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+secondary+antibodies/Goat+anti-mouse+IgG+(H%2BL)%2C+HRP+conjugate/pm42826891-109-3-7
Average 97 stars, based on 5898 article reviews
Hrp Conjugated Secondary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 5898 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+secondary+antibodies/Goat+anti-mouse+IgG+(H%2BL)%2C+HRP+conjugate/pm42826891-109-3-7
Average 97 stars, based on 5898 article reviews
hrp conjugated secondary antibodies - by Bioz Stars,
2026-10
97/100 stars
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Incubation:Article Title: Customizable virus-like particles deliver CRISPR-Cas9 ribonucleoprotein for effective ocular neovascular and Huntington's disease gene therapy. Article Snippet: .. HTT was detected using a mouse anti-huntingtin antibody (1:1,000, Santa Cruz Biotechnology, #sc-47757) followed by incubation with Article Title: Disrupted copper homeostasis promotes osteopontin-mediated hepatocyte-stellate cell crosstalk in Wilson disease. Article Snippet: Membranes were blocked with 5% non-fat milk and incubated overnight at 4 ◦C with the following primary antibodies anti-SPP1 (1:1000, Cat. No. 21933–1-AP, Proteintech), anti-ATP7B (1:1000, Cat. No. 19786–1-AP, Proteintech, with an expected molecular weight of approximately 157 kDa), anti Na+/K+-ATPase (1:1000, Cat. No. 3010, Cell Signaling Technology), and anti-β-actin (1:5000, Cat. No. 66009–1-Ig, Proteintech), which served as the loading control. .. After incubation with HRP-conjugated goat anti-rabbit or Article Title: The role of DNAJC3 in enhancing glioma progression and regulating the tumor immune microenvironment Article Snippet: After blocking the membrane for 1 h in Tris-buffered saline with Tween-20 containing 5% nonfat milk, the membrane was incubated overnight at 4 °C with primary antibodies specific for DNAJC3 (1:1000 dilution, 26721-1-AP; Proteintech), GAPDH (1:2000 dilution, 60004-1-Ig; Proteintech), GRP78 (1:2000 dilution, 66574-1-Ig; Proteintech), AKT (1:1000 dilution, 10176-2-AP; Proteintech), p-AKT (1:1000 dilution, 66444-1-Ig; Proteintech), ERK (1:1000 dilution, 67170-1-Ig; Proteintech), and p-ERK (1:1000 dilution, 28733-1-AP; Proteintech). .. Subsequently, the membrane was washed and incubated with anti-rabbit or Article Title: PLEKHA4 knockdown induces apoptosis in melanoma cells through the MAPK and β‑catenin signaling pathways. Article Snippet: Malignant melanoma (MM) is a highly aggressive subtype of skin cancer characterized by a poor prognosis, particularly in the advanced stages. despite advancements in targeted therapy and immunotherapy, the survival rates for MM remain low, underscoring the need for new therapeutic targets.. Pleckstrin homology domain‐containing family a member 4 (PleKHa4), which has regulatory functions in pivotal cellular processes, has emerged as a potential target in melanoma.. The present study aimed to investigate the role of PLEKHA4 in melanoma progression, focusing on its influ‐ ence on the MaPK and Wnt/β‐catenin signaling pathways. Article Title: CPNE7 Regulates Amyloidogenesis Through CAP1-Dependent ADAM10 Translation. Article Snippet: The antibodies used included CPNE7 (Proteintech, #17396- 1- AP, 1:1000), BACE1 (Abcam, #ab2077, 1:1000), APP- full length and β- CTF, α- CTF (Sigma, #A8717, 1:1000), β- CTF (6E10, Covance, 1:1000), ADAM10 (Proteintech, #25900- 1- AP, 1:1000), PD- L1 (Proteintech, #66248- 1- Ig, 1:1000), IDE (Proteintech, #21728- 1- AP, 1:1000), CAP1 (Proteintech, #68207- 1- Ig, 1:1000), and GAPDH (Proteintech, #60004- 1- Ig, 1:10000). .. After washing, the membranes were incubated for 1 h with HRP- conjugated anti- rabbit (Proteintech, #SA00001- 2, 1:5000) or Article Title: Rapamycin suppresses small bowel adenocarcinoma HUTU 80 cells proliferation by inhibiting hypoxia-inducible factor-1α mediated metabolic reprogramming Article Snippet: The samples were transferred to a polyvinylidene difluoride membrane, blocked with rapid blocking buffer (cat. No. P0252; Beyotime Institute of Biotechnology) for 15 minutes at 4 °C, incubated overnight with antibodies including cyclin-dependent kinases 2 (CDK2) (cat. No. 60312-1-Ig; ProteinTech Group, diluted 1:1500), mTOR (cat. No. AF6308; Affinity Biosciences, diluted 1:1500), phosphorylated (p)-mTOR (cat. No. AF3308; Affinity Biosciences, diluted 1:1500), 70 kDa ribosomal protein S6 kinase (p70S6k) (cat. No. AF6226; Affinity Biosciences, diluted 1:1500), p-p70S6k (cat. No. AF3228; Affinity Biosciences, diluted for 1:1500), eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) (cat. No. AF6432; Affinity Biosciences, diluted 1:1500), p-4E-BP1 (cat. No. AF3830; Affinity Biosciences, diluted 1:1500), HIF-1α (cat. No. BF8002; ProteinTech Group, diluted 1:10000), and β-actin (cat. No. 66009-1-lg; ProteinTech Group, diluted 1:1500). .. The membranes were washed three times with PBS-0.05% Tween 20, followed by incubation at room temperature for 2 hours with horseradish-peroxidase-conjugated anti-rabbit (cat. No. SA00001-2; ProteinTech Group, diluted 1:10000) or Article Title: Customizable virus-like particles deliver CRISPR–Cas9 ribonucleoprotein for effective ocular neovascular and Huntington’s disease gene therapy Article Snippet: .. HTT was detected using a mouse anti-huntingtin antibody (1:1,000, Santa Cruz Biotechnology, #sc-47757) followed by incubation with Membrane:Article Title: The role of DNAJC3 in enhancing glioma progression and regulating the tumor immune microenvironment Article Snippet: After blocking the membrane for 1 h in Tris-buffered saline with Tween-20 containing 5% nonfat milk, the membrane was incubated overnight at 4 °C with primary antibodies specific for DNAJC3 (1:1000 dilution, 26721-1-AP; Proteintech), GAPDH (1:2000 dilution, 60004-1-Ig; Proteintech), GRP78 (1:2000 dilution, 66574-1-Ig; Proteintech), AKT (1:1000 dilution, 10176-2-AP; Proteintech), p-AKT (1:1000 dilution, 66444-1-Ig; Proteintech), ERK (1:1000 dilution, 67170-1-Ig; Proteintech), and p-ERK (1:1000 dilution, 28733-1-AP; Proteintech). .. Subsequently, the membrane was washed and incubated with anti-rabbit or Saline:Article Title: PLEKHA4 knockdown induces apoptosis in melanoma cells through the MAPK and β‑catenin signaling pathways. Article Snippet: Malignant melanoma (MM) is a highly aggressive subtype of skin cancer characterized by a poor prognosis, particularly in the advanced stages. despite advancements in targeted therapy and immunotherapy, the survival rates for MM remain low, underscoring the need for new therapeutic targets.. Pleckstrin homology domain‐containing family a member 4 (PleKHa4), which has regulatory functions in pivotal cellular processes, has emerged as a potential target in melanoma.. The present study aimed to investigate the role of PLEKHA4 in melanoma progression, focusing on its influ‐ ence on the MaPK and Wnt/β‐catenin signaling pathways. |